The Antiaging Effect of Active Fractions and Ent-11α-Hydroxy-15-Oxo-Kaur-16-En-19-Oic Acid Isolated from Adenostemma lavenia (L.) O. Kuntze at the Cellular Level.
Irmanida BatubaraRika Indri AstutiMuhammad Eka PrastyaAuliya IlmiawatiMiwa MaedaMayu SuzukiAkie HamamotoHiroshi TakemoriPublished in: Antioxidants (Basel, Switzerland) (2020)
A. lavenia water and chloroform fractions showed antioxidant properties in vitro. The A. lavenia extracts and 11αOH-KA conferred resistance to H2O2 to S. pombe and B16F10 cells and extended the yeast lifespan in a concentration-dependent manner. These materials maintained the yeast mitochondrial activity, even in a high-glucose medium, and induced an antioxidant gene program, the transcriptional factor pap1+ and its downstream ctt1+. Accordingly, 11αOH-KA activated the antioxidative transcription factor NF-E2-related factor 2, NRF2, the mammalian ortholog of pap1+, in B16F10 cells, which was accompanied by enhanced hemeoxygenase expression levels. These results suggest that 11αOH-KA and A. lavenia extracts may protect yeast and mammalian cells from oxidative stress and aging. Finally, we hope that these materials could be helpful in treating COVID-19 patients, because A. lavenia extracts and NRF2 activators have been reported to alleviate the symptoms of pneumonia in model animals.
Keyphrases
- oxidative stress
- induced apoptosis
- high glucose
- diabetic rats
- transcription factor
- endothelial cells
- cell cycle arrest
- dna damage
- ischemia reperfusion injury
- poor prognosis
- signaling pathway
- anti inflammatory
- endoplasmic reticulum stress
- cell death
- gene expression
- cell wall
- sars cov
- depressive symptoms
- long non coding rna
- genome wide
- drug induced
- pi k akt
- dna methylation
- dna binding
- copy number
- quality improvement
- genome wide identification
- cell proliferation
- extracorporeal membrane oxygenation