CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma.
Yuehong ShenYuling ChenYuntao LinYicun LiPengfei LiuBiru ZhangYufan WangKing-Chi ChanNai-Ki MakMichael KahnRobert Zhong QiHongyu YangPublished in: Cell death & disease (2023)
In oral squamous cell carcinoma (OSCC), a highly aggressive and frequently lethal malignancy, the role and action mechanism of the microtubule regulatory protein CDK5RAP2 have not been fully understood. Here, we show that CDK5RAP2 is highly expressed in OSCC and its expression correlates with clinical stage and lymph node metastasis of the disease. The expression of CDK5RAP2 is regulated by the Wnt signaling pathway. Depletion of CDK5RAP2 inhibits the tumorigenesis and migration of OSCC cells and alters the OSCC cancer stem (-like) cell (CSC) signature. Notably, suppression of CDK5RAP2 expression disrupts spindle orientation during mitosis. Collectively, these results identify CDK5RAP2 as a potential CSC marker and reveal a mechanism that controls the CSC population in OSCC.
Keyphrases
- induced apoptosis
- signaling pathway
- cell cycle
- oxidative stress
- lymph node metastasis
- poor prognosis
- cell proliferation
- papillary thyroid
- stem cells
- pi k akt
- binding protein
- squamous cell carcinoma
- single cell
- transcription factor
- dna methylation
- mesenchymal stem cells
- bone marrow
- risk assessment
- small molecule
- protein protein
- amino acid
- human health
- cell cycle arrest