Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob ( Zea mays L.) Extract and In Vitro Evaluation of Anti-Inflammatory Effects.
Nattawadee KanpipitNatsajee NualkaewWorawikunya KiatponglarpAroonsri PripremSuthasinee ThapphasaraphongPublished in: Pharmaceutics (2022)
Sericin-alginate hydrogel formulations with purple waxy corn ( Zea mays L.) cob extract (PWCC) for topical anti-inflammatory application are developed and evaluated. The physical properties such as viscosity, pH, and anthocyanin release are examined and in vitro anti-inflammatory activities, such as NO inhibition and IL-6, IL-1β, TNF-α, iNOS, and COX-2 expression, are evaluated in LPS-stimulated RAW 264.7 murine macrophages. The sericin-alginate hydrogel is prepared by physical crosslinking through the ionic interaction of the polymers combined with anthocyanin from PWCC at pH 6.5. The hydrogel formulation with 2.00% w / v sericin, 0.20% w / v alginate, and 0.15% w / v PWCC (SN6) shows a suitable viscosity for topical treatment, the highest nitric oxide inhibition (79.43%), no cytotoxicity, and reduced expression of IL-6, IL-1β, and TNF-α mediators. Moreover, the SN6 formulation displays a sustained anthocyanin release over 8-12 h, which correlates with the Korsmeyer-Peppas model. The FT-IR spectrum of SN6 confirmed interaction of the sericin polymer with anthocyanins from PWCC via H-bonding by the shifted peak of amide I and amide III. In addition, the anthocyanin is stable in sericin hydrogels under heating-cooling storage conditions. Therefore, we suggest that this hydrogel formulation has potential as an anti-inflammatory agent. The formulation will be further investigated for in vivo studies and clinical trials in the future.
Keyphrases
- anti inflammatory
- drug delivery
- wound healing
- tissue engineering
- hyaluronic acid
- nitric oxide
- poor prognosis
- clinical trial
- drug release
- rheumatoid arthritis
- physical activity
- mental health
- mass spectrometry
- nitric oxide synthase
- binding protein
- inflammatory response
- long non coding rna
- atomic force microscopy
- study protocol
- smoking cessation
- open label
- case control
- phase iii