Narrow Leafed Lupin ( Lupinus angustifolius L.) β-Conglutin Seed Proteins as a New Natural Cytotoxic Agents against Breast Cancer Cells.
Julia Escudero-FeliuMaría García-CostelaSara Moreno-SanJuanJose D Puentes-PardoSandra Ríos ArrabalPaula González-NovoaMaría Isabel NúñezÁngel CarazoJose C Jimenez-LopezJosefa LeónPublished in: Nutrients (2023)
Breast cancer (BC) is the most widespread tumor in women and the second type of most common cancer worldwide. Despite all the technical and medical advances in existing therapies, between 30 and 50% of patients with BC will develop metastasis, which contributes to the failure of existing treatments. This situation urges the need to find more effective prevention and treatment strategies like the use of plant-based nutraceutical compounds. In this context, we purified three Narrow Leafed Lupin (NLL) β-conglutins isoforms using affinity-chromatography and evaluated their effectiveness in terms of viability, proliferation, apoptosis, stemness properties, and mechanism of action on both BC cell lines and a healthy one. NLL β-conglutins proteins have very promising effects at the molecular level on BC cells at very low concentrations, emerging as a potential natural cytotoxic agent and preserving the viability of healthy cells. These proteins could act through a dual mechanism involving tumorigenic and stemness-related genes such as SIRT1 and FoxO1, depending on the state of p53. More studies must be carried out to completely understand the underlying mechanisms of action of these nutraceutical compounds in BC in vitro and in vivo, and their potential use for the inhibition of other cancer cell types.
Keyphrases
- cell cycle arrest
- induced apoptosis
- signaling pathway
- pi k akt
- stem cells
- endoplasmic reticulum stress
- cell death
- oxidative stress
- epithelial mesenchymal transition
- breast cancer cells
- randomized controlled trial
- systematic review
- mass spectrometry
- human health
- transcription factor
- high speed
- ischemia reperfusion injury
- cell proliferation
- risk assessment
- adipose tissue
- squamous cell carcinoma
- polycystic ovary syndrome
- high resolution
- ms ms
- insulin resistance
- case control