Organosulfates (OSs) are well-known and ubiquitous constituents of atmospheric aerosol particles and have been used as secondary organic aerosol markers in many field studies. Hence, it is imperative to understand the formation of OS species in the atmosphere. Recently, hydroxy acids (HAs) and hydroxy acid sulfates have been extensively detected in the atmospheric environment. However, the reaction mechanism of HAs to form OSs is much less understood. In this work, we have mainly investigated the reaction of typical α-HAs, including glycolic acid (GA) and lactic acid (LA), and SO 3 at the liquid aerosol surface using quantum chemistry calculations and Born-Oppenheimer molecular dynamics simulations. The OH group orientation of α-HAs at the air-water interface is found to exert a significant impact on the formation of OSs. The OH group pointing to the gas phase is obviously beneficial to the formation of OSs. Two key factors are discovered important to the reaction of α-HAs adsorbed on the liquid surface with SO 3 : (a) the exposure position of the active site to the gas phase and (b) the reactivity of the exposed site to the attracted SO 3 molecule. Moreover, we found that the air-water interface exerts a significant influence on the physicochemical behaviors of GA and LA, especially on their OH group orientation, and thus leads to their different properties for the SO 3 colliding reaction. The presented reaction mechanism provides a new feasible pathway for the production of OSs at the liquid aerosol surface, which may have important impacts on the formation of organic aerosols.