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MASH Native: A Unified Solution for Native Top-Down Proteomics Data Processing.

Eli J LarsonMelissa R PergandeMichelle E MossKalina J RosslerR Kent WengerBoris KrichelHarini JosyerJake A MelbyDavid S RobertsKyndalanne PikeZhuoxin ShiHsin-Ju ChanBridget KnightHolden T RogersKyle A BrownIrene M OngKyowon JeongMichael Thomas MartySean J McIlwainYing Ge
Published in: bioRxiv : the preprint server for biology (2023)
Native top-down proteomics (nTDP) integrates native mass spectrometry (nMS) with top-down proteomics (TDP) to provide comprehensive analysis of protein complexes together with proteoform identification and characterization. Despite significant advances in nMS and TDP software developments, a unified and user-friendly software package for analysis of nTDP data remains lacking. Herein, we have developed MASH Native to provide a unified solution for nTDP to process complex datasets with database searching capabilities in a user-friendly interface. MASH Native supports various data formats and incorporates multiple options for deconvolution, database searching, and spectral summing to provide a one-stop shop for characterizing both native protein complexes and proteoforms. The MASH Native app, video tutorials, written tutorials and additional documentation are freely available for download at https://labs.wisc.edu/gelab/MASH_Explorer/MASHNativeSoftware.php . All data files shown in user tutorials are included with the MASH Native software in the download .zip file.
Keyphrases
  • mass spectrometry
  • electronic health record
  • data analysis
  • magnetic resonance imaging
  • liquid chromatography
  • emergency department
  • adverse drug
  • small molecule
  • protein protein
  • deep learning
  • tandem mass spectrometry