Direct on-the-spot detection of SARS-CoV-2 in patients.
Nadav Ben-AssaRawi NaddafTal GefenTal CapuchaHaitham HajjoNoa MandelbaumLilach ElbaumPeter RogovDaniel A KingShai KaplanAssaf RotemMichal ChowersMoran Szwarcwort-CohenMical PaulNaama Geva-ZatorskyPublished in: Experimental biology and medicine (Maywood, N.J.) (2020)
Humanity is currently experiencing a global pandemic with devastating implications on human health and the economy. Most countries are gradually exiting their lockdown state. We are currently lacking rapid and simple viral detections, especially methods that can be performed in the household. Here, we applied RT-LAMP directly on human clinical swabs and self-collected saliva samples. We adjusted the method to allow simple and rapid viral detection, with no RNA purification steps. By testing our method on over 180 human samples, we determined its sensitivity, and by applying it to other viruses, we determined its specificity. We believe this method has a promising potential to be applied world-wide as a simple and cheap surveillance test for SARS-CoV-2.
Keyphrases
- sars cov
- loop mediated isothermal amplification
- human health
- endothelial cells
- respiratory syndrome coronavirus
- risk assessment
- end stage renal disease
- sensitive detection
- ejection fraction
- newly diagnosed
- chronic kidney disease
- pluripotent stem cells
- induced pluripotent stem cells
- climate change
- public health
- prognostic factors
- peritoneal dialysis
- real time pcr