Uncovering the interference from lipid fragments on the qualification and quantification of serum metabolites in matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis.

The phospholipids could produce fragment ions through ISD and PSD in MALDI MS with both nanoparticle and organic matrices. The fragments exerted influence on the qualification and qualification of metabolites in serum. By choosing the proper extraction method, the interference from lipid fragments could be efficiently alleviated.