A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells.
Enrica FabbriAnna TamaniniTiziana JakovaJessica GasparelloAlex ManicardiRoberto CorradiniGiuseppe SabbioniAlessia FinottiMonica BorgattiIlaria LamprontiSilvia MunariMaria Cristina DechecchiGiulio CabriniRoberto GambariPublished in: Molecules (Basel, Switzerland) (2017)
Peptide nucleic acids (PNAs) are very useful tools for gene regulation at different levels, but in particular in the last years their use for targeting microRNA (anti-miR PNAs) has provided impressive advancements. In this respect, microRNAs related to the repression of cystic fibrosis transmembrane conductance regulator (CFTR) gene, which is defective in cystic fibrosis, are of great importance in the development of new type of treatments. In this paper we propose the use of an anti-miR PNA for targeting miR-145, a microRNA reported to suppress CFTR expression. Octaarginine-anti-miR PNA conjugates were delivered to Calu-3 cells, exerting sequence dependent targeting of miR-145-5p. This allowed to enhance expression of the miR-145 regulated CFTR gene, analyzed at mRNA (RT-qPCR, Reverse Transcription quantitative Polymerase Chain Reaction) and CFTR protein (Western blotting) level.
Keyphrases
- cystic fibrosis
- long non coding rna
- poor prognosis
- cell proliferation
- pseudomonas aeruginosa
- nucleic acid
- long noncoding rna
- lung function
- induced apoptosis
- binding protein
- cancer therapy
- cell cycle arrest
- copy number
- genome wide
- signaling pathway
- gene expression
- cell death
- oxidative stress
- dna methylation
- south africa
- endoplasmic reticulum stress
- mass spectrometry
- drug induced