Honey bees provide essential pollination services to the terrestrial ecosystem and produce important agricultural products. As a beneficial lactic acid bacterium, Enterococcus faecium is often supplied as a probiotic for honey bees and other animals. However, the underlying mechanisms of its actions and possible safety risks are not well understood. We present the first complete genome sequence of E. faecium isolated from the honey bee gut using nanopore sequencing, and investigate the effects and mechanisms of interactions between E. faecium and honey bees via transcriptome and miRNA analysis. E. faecium colonization increased honey bee gut weight. Transcriptome analysis showed that developmental genes were up-regulated. In accordance, the target genes of the down-regulated miRNAs were enriched in developmental pathways. We describe how E. faecium increases honey bee gut weight at the transcriptional and post-transcriptional levels, and add insights about how miRNAs mediate host and bacteria interactions.
Keyphrases
- genome wide
- transcription factor
- gene expression
- lactic acid
- climate change
- body mass index
- human health
- weight loss
- healthcare
- physical activity
- primary care
- risk assessment
- single cell
- mental health
- genome wide identification
- escherichia coli
- dna methylation
- bioinformatics analysis
- single molecule
- staphylococcus aureus
- oxidative stress
- cystic fibrosis
- pseudomonas aeruginosa
- body weight