Expression of a bacterial 3-dehydroshikimate dehydratase (QsuB) reduces lignin and improves biomass saccharification efficiency in switchgrass (Panicum virgatum L.).
Zhangying HaoSasha YogiswaraTong WeiVeronica Teixeira BenitesAnagh SinhaGeorge WangEdward E K BaidooPamela C RonaldHenrik V SchellerDominique LoquéAymerick EudesPublished in: BMC plant biology (2021)
This study validates the transfer of the QsuB engineering approach from a model plant to switchgrass. We have demonstrated altered expression of two important traits: lignin content and accumulation of a co-product. We found that the choice of promoter to drive QsuB expression should be carefully considered when deploying this strategy to other bioenergy crops. Field-testing of engineered QsuB switchgrass are in progress to assess the performance of the introduced traits and agronomic performances of the transgenic plants.