Reinforced Poly(ε-caprolactone) Bimodal Foams via Phospho-Calcified Cellulose Nanowhisker for Osteogenic Differentiation of Human Mesenchymal Stem Cells.
Alexander P LyubartsevMohsen ShahrousvandBabak KaffashiPublished in: ACS biomaterials science & engineering (2018)
In this work, phospho-calcified cellulose nanowhiskers (PCCNWs) were prepared from wastepaper powder (WPP) and were dispersed in poly(ε-caprolactone) (PCL). The biocompatible and biodegradable (PCL)/PCCNW bimodal foam nanocomposites with two species cell sizes were prepared by the solvent casting/particulate leaching method in different weight percentage of PCCNWs. The mechanical, thermal, and in vitro biological properties of PCL/PCCNW nanocomposites were investigated. All PCL/PCCNW scaffolds were hydrophilic, biodegradable, and also noncytotoxic. The human mesenchymal stem cells were cultured on the prepared PCL/PCCNW bimodal foam nanocomposites and differentiated to osteoblasts. On the basis of evaluating tests such as MTT assay, acridine orange/ethidium bromide staining, alkaline phosphatase assay, calcium content assay, and alizarin red staining, PCL/PCCNW scaffolds were introduced as an appropriate option for emulating the behavior of extracellular matrix. Increasing PCCNWs improves the mechanical, hydrophilic, and biodegradability properties of the nanocomposites as well as their osteoconductivity.
Keyphrases
- mesenchymal stem cells
- endothelial cells
- extracellular matrix
- tissue engineering
- ionic liquid
- reduced graphene oxide
- high throughput
- carbon nanotubes
- drug delivery
- umbilical cord
- cell therapy
- liquid chromatography
- body mass index
- single cell
- induced pluripotent stem cells
- physical activity
- pluripotent stem cells
- visible light
- weight loss
- mass spectrometry
- weight gain
- high resolution
- tandem mass spectrometry