Single cell atlas for 11 non-model mammals, reptiles and birds.
Dongsheng ChenJian SunJiacheng ZhuXiangning DingTianming LanXiran WangWeiying WuZhihua OuLinnan ZhuPeiwen DingHaoyu WangLihua LuoRong XiangXiaoling WangJiaying QiuShiyou WangHaimeng LiChaochao ChaiLangchao LiangFuyu AnLe ZhangLei HanYixin ZhuFeiyue WangYuting YuanWendi WuChengcheng SunHaorong LuJihong WuXinghuai SunShenghai ZhangSunil Kumar SahuPing LiuJun XiaLijing ZhangHaixia ChenDongming FangYuying ZengYiquan WuZehua CuiQian HeSanjie JiangXiaoyan MaWeimin FengYan XuFang LiZhongmin LiuLei ChenFang ChenXin JinWei QiuTianjiao WangYang LiXiumei XingHuanming YangYanchun XuYan HuaYahong LiuHuan LiuXue LiuPublished in: Nature communications (2021)
The availability of viral entry factors is a prerequisite for the cross-species transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Large-scale single-cell screening of animal cells could reveal the expression patterns of viral entry genes in different hosts. However, such exploration for SARS-CoV-2 remains limited. Here, we perform single-nucleus RNA sequencing for 11 non-model species, including pets (cat, dog, hamster, and lizard), livestock (goat and rabbit), poultry (duck and pigeon), and wildlife (pangolin, tiger, and deer), and investigated the co-expression of ACE2 and TMPRSS2. Furthermore, cross-species analysis of the lung cell atlas of the studied mammals, reptiles, and birds reveals core developmental programs, critical connectomes, and conserved regulatory circuits among these evolutionarily distant species. Overall, our work provides a compendium of gene expression profiles for non-model animals, which could be employed to identify potential SARS-CoV-2 target cells and putative zoonotic reservoirs.
Keyphrases
- sars cov
- single cell
- respiratory syndrome coronavirus
- rna seq
- induced apoptosis
- high throughput
- poor prognosis
- cell cycle arrest
- genome wide
- transcription factor
- lymph node
- public health
- endoplasmic reticulum stress
- binding protein
- dna methylation
- signaling pathway
- mass spectrometry
- genome wide identification
- cell death
- bioinformatics analysis
- genome wide analysis
- cell therapy
- pi k akt
- high speed
- atomic force microscopy