Dexamethasone attenuated thoracic aortic aneurysm and dissection in vascular smooth muscle cell Tgfbr2-disrupted mice with CCL8 suppression.
Xipeng WangQingle LiWei LiTao ZhangXiaoyan LiYang JiaoXuemin ZhangJingjun JiangXiaoping ZhangXiaoming ZhangPublished in: Experimental physiology (2022)
male mice were used to build a TAAD mouse model, and bioinformatic analyses revealed enriched inflammatory signal pathways and upregulated chemokine (C-C motif) ligand 8 (CCL8). So we hypothesized that vascular smooth muscle cell (VSMC) Tgfbr2 disruption in postnatal mice results in aortic inflammation associated with CCL8 secretion. Real-time quantitative PCR and serum enzyme-linked immunosorbent assay (ELISA) results confirmed that CCL8 expression began to increase after VSMC Tgfbr2 disruption. Next, we cultured mouse thoracic aortas ex vivo, and observed that the protein expression of CCL8 in culture supernatants was increased by ELISA. Subsequently, the co-localization of CCL8 with α-smooth muscle actin or CD68 was found to be significantly increased by immunofluorescence. Then, dexamethasone (DEX) was used to treat TAAD in VSMC Tgfbr2-disrupted mice; the results of histochemical, immunofluorescence and immunohistochemical staining indicated that DEX therapy reduced CCL8 secretion, inflammatory cell recruitment, aortic medial thickening, elastic fibre fragmentation, extracellular matrix degradation and contractile apparatus impairment, and thereby ameliorated TAAD formation. Western blotting showed that mitogen-activated protein kinase and nuclear factor-κB signalling pathways in aorta were overactivated after VSMC Tgfbr2 disruption, but inhibited by DEX therapy. Altogether, CCL8 might be an important promoter in TAAD formation of VSMC Tgfbr2-disrupted mice, and DEX provided potential therapeutic effects in TAAD treatment.
Keyphrases
- smooth muscle
- liver fibrosis
- liver injury
- single cell
- drug induced
- extracellular matrix
- nuclear factor
- oxidative stress
- high fat diet induced
- cell therapy
- aortic valve
- mouse model
- low dose
- toll like receptor
- gene expression
- heart failure
- endothelial cells
- preterm infants
- skeletal muscle
- poor prognosis
- high resolution
- insulin resistance
- adipose tissue
- coronary artery
- mesenchymal stem cells
- south africa
- spinal cord injury
- transcription factor
- binding protein
- combination therapy