Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts.

Laiyou WangAihua DengYun ZhangShuwen LiuYong LiangHua BaiDi CuiQidi QiuXiuling ShangZhao YangXiuping HeTingyi Wen

Published in: Biotechnology for biofuels (2018)

An efficient and versatile multiplex genome editing method was developed in yeasts. The method would provide an efficient toolkit for genetic engineering and synthetic biology researches of O. polymorpha and other yeast species.

Keyphrases

genome editing
crispr cas
saccharomyces cerevisiae
high throughput
gene expression