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The Action of the Pulsed Electric Field of the Subnanosecond Range on Human Tumor Cells.

Aleksey A PetrovAnastasiya A MoralevaNadezhda V AntipovaRavil Kh AmirovIgor S SamoylovSergey Y Savinov
Published in: Bioelectromagnetics (2022)
The action of the pulsed electric field of the subnanosecond range on Jurkat, HEK 293, and U-87 MG human cell lines was studied. The cells were treated in a waveguide in 0.18 ml electrodeless Teflon cuvettes. The electric field strength in the cell culture medium was ~2 kV/cm, the pulse duration was ~1 ns, the leading edge was 150 ps, the frequency was 100 Hz, and the treatment time was 5 min. According to estimates, the change of the transmembrane potential during the pulse was ~20 mV and we assume that it was insufficient for electroporation. Jurkat and HEK 293 cells appeared to be more resistant to the treatment than U-87 MG cells. We have observed that the impulses with the above-mentioned parameters can cause a noticeable change in the mitochondrial activity of U-87 MG cells. © 2022 Bioelectromagnetics Society.
Keyphrases
  • induced apoptosis
  • cell cycle arrest
  • endothelial cells
  • oxidative stress
  • endoplasmic reticulum stress
  • cell death
  • combination therapy
  • pi k akt
  • zika virus
  • replacement therapy
  • pluripotent stem cells