Assessment of Antibiotic Resistance and Efflux Pump Gene Expression in Neisseria Gonorrhoeae Isolates from South Africa by Quantitative Real-Time PCR and Regression Analysis.

Nireshni Mitchev Ravesh Singh Veron Ramsuran Arshad Ismail Mushal Allam Stanford Kwanda Florah Mnyameni Sharana Mahomed Khine Swe Swe-Han Abraham J Niehaus Koleka P Mlisana

Published in: International journal of microbiology (2022)

Antimicrobial stewardship includes exploring options to conserve currently available drugs for gonorrhoea treatment. There is the potential to predict an isolate as either susceptible or nonsusceptible based on the mRNA expression level of specific candidate markers, to inform patient management. This real-time qPCR approach, with few targets, can be further investigated for use as a potentially cost-effective diagnostic tool to detect resistance.

Keyphrases

real time pcr
gene expression
south africa
men who have sex with men
dna methylation
hiv positive
case report
high resolution
risk assessment
atomic force microscopy
human health
hepatitis c virus
climate change
single molecule
smoking cessation