Derivation and propagation of spermatogonial stem cells from human pluripotent cells.

Huiming XuMengbo YangRuhui TianYonghui WangLinhong LiuZijue ZhuShi YangQingqing YuanMinghui NiuChencheng YaoErlei ZhiPeng LiChenhao ZhouZuping HeZheng LiWei-Qiang Gao

Published in: Stem cell research & therapy (2020)

SSCLCs were successfully derived from hPSCs and propagated for a long time. The SSCLCs resembled their counterpart human GPR125+ spermatogonia, as evidenced by the grape-like morphology, transcriptome, homing, and functional characteristics. Therefore, hPSC-derived SSCLCs may provide a reliable cell source for studying human SSCs biological properties, disease modeling, and drug toxicity screening.

Keyphrases

endothelial cells
induced pluripotent stem cells
pluripotent stem cells
oxidative stress
emergency department
stem cells
dna methylation
cell death
genome wide