Single-stranded DNA structural diversity: TAGGGT from monomers to dimers to tetramer formation.

Jacob E PorterPrem ChapagainFrancisco Fernandez Lima

Published in: Rapid communications in mass spectrometry : RCM (2019)

This is the first observation of multiple conformations of TAGGGT complexes (n = 1, 2 and 4) in 10 mM ammonium acetate. Candidate structures with intramolecular interactions of the form of G+ -G and traditional A-T base pairing agreed with the experimental trends. Our results demonstrate the structural diversity of TAGGGT monomers, dimers and tetramers in the gas phase beyond the previously reported solution structure, using 10 mM ammonium acetate to replicate biological conditions.

Keyphrases

ionic liquid
circulating tumor
high resolution
single molecule
nucleic acid
binding protein
energy transfer