Login / Signup

Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub.

Shi-Wei ZhaoJing-Fang GuoLei KongShuai NieXue-Mei YanTian-Le ShiXue-Chan TianHai-Yao MaYu-Tao BaoZhi-Chao LiZhao-Yang ChenRen-Gang ZhangYong-Peng MaEl-Kassaby YousryIlga M PorthWei ZhaoJian-Feng Mao
Published in: Scientific data (2023)
Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.
Keyphrases
  • genome wide
  • copy number
  • dna methylation
  • oxidative stress
  • gene expression
  • small molecule
  • machine learning
  • single cell
  • artificial intelligence
  • transcription factor
  • genetic diversity