Staphylococcus aureus Bacteriophage 52 Endolysin Exhibits Anti-Biofilm and Broad Antibacterial Activity Against Gram-Positive Bacteria.
Mujib Abdulkadir Abdurahmanİnci DurukanTuba DinçerSerap PektaşErsin KarataşAli Osman KiliçPublished in: The protein journal (2023)
Bacteriophage endolysins have been shown to hold great promise as new antibacterial agents for animal and human health in food preservation. In the present study, endolysin from Staphylococcus aureus subsp. aureus ATCC 27692-B1 bacteriophage 52 (LysSA52) was cloned, expressed, and characterized for its antimicrobial properties. Following DNA extraction from bacteriophage 52, a 1446-bp DNA fragment containing the endolysin gene (lysSA52) was obtained by PCR amplification and cloned into pET SUMO expression vector. The positive clone was validated by sequencing and open-reading frame analysis. The LysSA52 sequence shared high homology with staphylococcal phage endolysins of the SA12, SA13, and DSW2 phages and others. The cloned lysSA52 gene encoding 481 amino acids endolysin was expressed in Escherichia coli BL21 with a calculated molecular mass of 66 kDa (LysSA52). This recombinant endolysin LysSA52 exhibited lytic activity against 8 of 10 Gram-positive bacteria via agar spot-on lawn antimicrobial assay, including methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Streptococcus pneumonia, Streptococcus pyogenes, Enterococcus faecium, Enterococcus faecalis, and Bacillus atrophaeus. In addition, the 0.50 mg/mL, LysSA52 endolysins reduced about 60% of the biofilms of S. aureus and S. epidermidis established on a microtiter plate in 12 h treatment. The data from this study indicate that LysSA52 endolysin could be used as an antibacterial protein to prevent and treat infections caused by staphylococci and several other Gram-positive pathogenic bacteria irrespective of their antibiotic resistance.
Keyphrases
- staphylococcus aureus
- biofilm formation
- methicillin resistant staphylococcus aureus
- human health
- pseudomonas aeruginosa
- escherichia coli
- candida albicans
- gram negative
- amino acid
- risk assessment
- single molecule
- genome wide
- copy number
- cell free
- circulating tumor
- dna methylation
- poor prognosis
- gene expression
- silver nanoparticles
- big data
- machine learning
- single cell
- nucleic acid
- climate change
- minimally invasive
- multidrug resistant
- anti inflammatory
- binding protein
- small molecule
- acute respiratory distress syndrome
- genome wide identification
- protein protein
- klebsiella pneumoniae