A novel whole-cell biosensor of Pseudomonas aeruginosa to monitor the expression of quorum sensing genes.
Chiqian ZhangDamien ParrelloPamela J B BrownJudy D WallZhiqiang HuPublished in: Applied microbiology and biotechnology (2018)
A novel whole-cell biosensor was developed to noninvasively and simultaneously monitor the in situ genetic activities of the four quorum sensing (QS) networks in Pseudomonas aeruginosa PAO1, including the las, rhl, pqs, and iqs systems. P. aeruginosa PAO1 is a model bacterium for studies of biofilm and pathogenesis while both processes are closely controlled by the QS systems. This biosensor worked well by selectively monitoring the expression of one representative gene from each network. In the biosensor, the promoter regions of lasI, rhlI, pqsA, and ambB (QS genes) controlled the fluorescent reporter genes of Turbo YFP, mTag BFP2, mNEON Green, and E2-Orange, respectively. The biosensor was successful in monitoring the impact of an important environmental factor, salt stress, on the genetic regulation of QS networks. High salt concentrations (≥ 20 g·L-1) significantly downregulated rhlI, pqsA, and ambB after the biosensor was incubated for 17 h to 18 h at 37 °C, resulting in slow bacterial growth.
Keyphrases
- pseudomonas aeruginosa
- label free
- genome wide
- sensitive detection
- quantum dots
- gold nanoparticles
- poor prognosis
- dna methylation
- cystic fibrosis
- genome wide identification
- copy number
- biofilm formation
- bioinformatics analysis
- staphylococcus aureus
- escherichia coli
- long non coding rna
- crispr cas
- genome wide analysis
- bone marrow
- stem cells
- mesenchymal stem cells
- living cells
- life cycle