Wearable Microneedle Patch For Colorimetric Detection Of Multiple Signature Biomarkers In Vivo Towards Diabetic Diagnosis.

Type 2 diabetes (T2D) is rapidly emerging as a global public health problem. While blood glucose monitoring has been the primary method of managing diabetes for decades, the increasing global prevalence of the disease suggests that there might be a need to identify additional biomarkers for a more precise early diagnosis. Herein, a microneedle patch (MN) based wearable sensor was developed for the purpose of diabetic diagnosis. Utilizing methacrylic acid modified gelatin and polyvinyl alcohol in the fabrication of MNs has improved their mechanical properties for skin penetration and increased swelling capacity for skin tissue fluid extraction, thanks to the double crosslinking mechanism. The fabricated MNs were further integrated with test paper functionalized with enzyme and dye molecules to detect multiple signature biomarkers related to T2D in vivo through a colorimetric reaction. Such an MN sensor holds significant promise for the real-time monitoring of various biomarker related to chronic diseases and aging. This article is protected by copyright. All rights reserved.