Deep oncopanel sequencing reveals within block position-dependent quality degradation in FFPE processed samples.
Yifan ZhangThomas M BlomquistRebecca KuskoDaniel StetsonZhihong ZhangLihui YinRobert SebraBinsheng GongJennifer S LococoVinay K MittalNatalia NovoradovskayaJi-Youn YeoNicole DominiakJennifer HippAmelia RaymondFujun QiuHanane AribMelissa L SmithJay E BrockDaniel H FarkasDaniel J CraigErin L CrawfordDan LiTom MorrisonNikola TomWenzhong XiaoMary YangChristopher E MasonTodd A RichmondWendell JonesDonald J JohannLeming ShiWeida TongJames C WilleyJoshua XuPublished in: Genome biology (2022)
To assure reliable results, we recommend avoiding the block surface portion and restricting mutation detection to genomic regions of high confidence.