A Metabarcoding Protocol to Analyze Coastal Planktic Communities Collected by Desalination Plant Filters: From Sampling to Bioinformatic Exploratory Analyses.
Matteo CecchettoAndrea Di CesareEster M EckertIsabella MoroDiego FontanetoStefano SchiaparelliPublished in: Methods in molecular biology (Clifton, N.J.) (2022)
The temporal dynamics of coastal planktic communities can be disclosed through DNA metabarcoding on the filters of reverse-osmosis desalination plants. Here, we describe the steps that are necessary to process the filters in order to create the subsamples used for DNA extraction and the bioinformatic pipeline to perform the first exploratory analyses on this kind of dataset.