hsa_circ_0087100/hsa-miR-6743-5p affects Th1 cell differentiation by regulating STAT1 in diabetic retinopathy.
Shuai HeDongwei LaiChenggong MaChunren MengChunyang CaiQian ChenChufeng GuQinghua QiuPublished in: Epigenomics (2024)
Objective: To elucidate the role of the competitive endogenous RNA (ceRNA) network in immune infiltration of diabetic retinopathy (DR). Methods: We obtained differentially expressed (DE) circRNAs, miRNAs and mRNAs from the Gene Expression Omnibus database. Then, we identified immune infiltration by CIBERSORT and single-sample gene set enrichment analysis and discovered co-expression genes by weighted gene co-expression network analysis. Furthermore, STAT1 -mediated Th1 differentiation was determined in DR cell models, DR patients and DR mouse models. Results: hsa_circ_0087100/hsa-miR-6743-5p/STAT1 was involved in immune infiltration of Th1 cells. Aberrant expression of the ceRNA network and STAT1 -mediated Th1 differentiation was thus verified in vitro and in vivo . Conclusion: hsa_circ_0087100/hsa-miR-6743-5p/STAT1 may affect Th1 cell differentiation in DR.
Keyphrases
- diabetic retinopathy
- network analysis
- editorial comment
- poor prognosis
- cell proliferation
- gene expression
- optical coherence tomography
- long non coding rna
- genome wide
- end stage renal disease
- newly diagnosed
- genome wide identification
- copy number
- induced apoptosis
- dna methylation
- magnetic resonance
- mouse model
- peritoneal dialysis
- magnetic resonance imaging
- genome wide analysis
- mesenchymal stem cells
- cell therapy
- cell death