Prototypical innate immune mechanism hijacked by leukemia-initiating mutant stem cells for selective advantage and immune evasion in Ptpn11-associated juvenile myelomonocytic leukemia.
Cheng-Kui QuHong ZhengPeng ZhaoZhenya TanWen-Mei YuJuwita WernerElliot StieglitzChristopher C PorterShanmuganathan ChandrakasanDaniel WechslerSimon Mendez-FerrerPublished in: Research square (2024)
Juvenile myelomonocytic leukemia (JMML), a clonal hematologic malignancy, originates from mutated hematopoietic stem cells (HSCs). The mechanism sustaining the persistence of mutant stem cells, leading to leukemia development, remains elusive. In this study, we conducted comprehensive examination of gene expression profiles, transcriptional factor regulons, and cell compositions/interactions throughout various stages of tumor cell development in Ptpn11 mutation-associated JMML. Our analyses revealed that leukemia-initiating Ptpn11 E76K/+ mutant stem cells exhibited de novo activation of the myeloid transcriptional program and aberrant developmental trajectories. These mutant stem cells displayed significantly elevated expression of innate immunity-associated anti-microbial peptides and pro-inflammatory proteins, particularly S100a9 and S100a8 . Biological experiments confirmed that S100a9/S100a8 conferred a selective advantage to the leukemia-initiating cells through autocrine effects and facilitated immune evasion by recruiting and promoting immune suppressive myeloid-derived suppressor cells (MDSCs) in the microenvironment. Importantly, pharmacological inhibition of S100a9/S100a8 signaling effectively impeded leukemia development from Ptpn11 E76K/+ mutant stem cells. These findings collectively suggest that JMML tumor-initiating cells exploit evolutionarily conserved innate immune and inflammatory mechanisms to establish clonal dominance.
Keyphrases
- stem cells
- bone marrow
- acute myeloid leukemia
- cell therapy
- induced apoptosis
- innate immune
- cell cycle arrest
- wild type
- gene expression
- transcription factor
- oxidative stress
- mesenchymal stem cells
- depressive symptoms
- poor prognosis
- endoplasmic reticulum stress
- microbial community
- cell death
- quality improvement
- dendritic cells
- genome wide
- cell proliferation
- immune response
- copy number
- heat stress