Replication of single-cell proteomics data reveals important computational challenges.
Christophe VanderaaLaurent GattoPublished in: Expert review of proteomics (2021)
Replicating SCP data analyses uncovers important challenges in SCP data analysis. We describe two such challenges in detail: batch correction and data missingness. We provide the current state-of-the-art and illustrate the associated limitations. We also highlight the intimate dependence that exists between batch effects and data missingness and offer avenues for dealing with these exciting challenges.