Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery.
Lourdes Lorigados PedreLilia Morales ChaconNancy Pavón FuentesMaría de Los A Robinson AgramonteTeresa Serrano SánchezRachel M Cruz-XenesMei-Li Díaz HungBárbara Estupiñán DíazMargarita M Báez MartínSandra Orozco-SuárezPublished in: Behavioral sciences (Basel, Switzerland) (2018)
Increasing amounts of evidence support the role of inflammation in epilepsy. This study was done to evaluate serum follow-up of IL-1β and IL-6 levels, as well as their concentration in the neocortex, and the relationship of central inflammation with NF-κB and annexin V in drug-resistant temporal lobe epileptic (DRTLE) patients submitted to surgical treatment. Peripheral and central levels of IL-1β and IL-6were measured by ELISA in 10 DRTLE patients. The sera from patients were taken before surgery, and 12 and 24 months after surgical treatment. The neocortical expression of NF-κB was evaluated by western blotting and annexin V co-localization with synaptophysin by immunohistochemistry. The neocortical tissues from five patients who died by non-neurological causes were used as control. Decreased serum levels of IL-1 and IL-6 were observed after surgery; at this time, 70% of patients were seizure-free. No values of IL-1 and IL-6 were detected in neocortical control tissue, whereas cytokine levels were evidenced in DRTLE. Increased NF-κB neocortex expression was found and the positive annexin V neurons were more obvious in the DRTLE tissue, correlating with IL-6 levels. The follow-up study confirmed that the inflammatory alterations disappeared one year after surgery, when the majority of patients were seizure-free, and the apoptotic death process correlated with inflammation.
Keyphrases
- drug resistant
- end stage renal disease
- ejection fraction
- oxidative stress
- prognostic factors
- signaling pathway
- minimally invasive
- poor prognosis
- immune response
- patient reported outcomes
- cell proliferation
- atrial fibrillation
- lps induced
- coronary artery disease
- acute coronary syndrome
- brain injury
- cystic fibrosis
- acinetobacter baumannii
- pseudomonas aeruginosa