A distinct linker mediating ER-autophagosome membrane contact site (EACS) in plants.
Hao YeJiayang GaoLiwen JiangPublished in: Autophagy (2023)
Endoplasmic reticulum (ER) membrane contacts play a central role in regulating autophagosome formation in yeast and mammals. However, a direct functional linkage between the ER and autophagosomes in plants remains elusive. We have recently identified and characterized a plant-unique protein complex consisting of AT4G22540/OSBP2A/ORP2A (oxysterol binding protein-related protein 2A), the ER resident protein AT3G60600/VAP27-1 (vesicle-associated protein 27-1) and AT2G45170/ATG8e (autophagy related 8e) that mediate the ER-autophagosome membrane contact site (EACS) in the model plant Arabidopsis thaliana . Knockdown (KD) of ORP2A affects autophagosome formation and seedling development, whereas ORP2A KD root cells show accumulation of the macroautophagic/autophagic core machinery and PtdIns3P in enlarged ER membranes under autophagy conditions. This study reveals the molecular architecture and functions of a distinct plant EACS in regulating autophagosome formation via lipid redistribution.
Keyphrases
- endoplasmic reticulum
- estrogen receptor
- arabidopsis thaliana
- binding protein
- cell death
- breast cancer cells
- induced apoptosis
- endoplasmic reticulum stress
- oxidative stress
- signaling pathway
- cell cycle arrest
- gene expression
- cell wall
- single molecule
- fatty acid
- hepatitis c virus
- men who have sex with men
- drug induced
- antiretroviral therapy