Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells.

Lisa J AlcockBruno L OliveiraMichael J DeeryTara Louise PukalaMichael V PerkinsGonçalo J L Bernardes Justin M Chalker

Published in: ACS chemical biology (2019)

Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

Keyphrases

living cells
fluorescent probe
induced apoptosis
single molecule
small molecule
cell cycle arrest
single cell
endoplasmic reticulum stress
signaling pathway
quantum dots
cell death
oxidative stress
hydrogen peroxide
cell proliferation
pi k akt
sensitive detection