Transcriptional regulation of microalgae for concurrent lipid overproduction and secretion.
Da-Wei LiSrinivasan BalamuruganYu-Feng YangJian-Wei ZhengDan HuangLi-Gong ZouWei-Dong YangJie-Sheng LiuYuanfang GuanHong-Ye LiPublished in: Science advances (2019)
Commercialization of algal lipids and biofuels is still impractical owing to the unavailability of lipogenic strains and lack of economically viable oil extraction strategies. Because lipogenesis is governed by multiple factors, success in generating industrial-suitable algal strains using conventional strategies has been limited. We report the discovery of a novel bZIP1 transcription factor, NobZIP1, whose overexpression results in a remarkable elevation of lipid accumulation and lipid secretion in a model microalga Nannochloropsis oceanica, without impairing other physiological properties. Chromatin immunoprecipitation-quantitative PCR analysis revealed that the key genes up- and down-regulated by NobZIP1 are involved in lipogenesis and cell wall polymer synthesis, respectively, which, in turn, induce lipid overproduction and secretion. Among these regulated genes, UDP-glucose dehydrogenase was shown to alter cell wall composition, thus also boosting lipid secretion. In summary, these results offer a comprehensive strategy for concurrent lipid overproduction and secretion, strongly increasing the commercial potential of microalgae.
Keyphrases
- transcription factor
- cell wall
- fatty acid
- genome wide
- genome wide identification
- escherichia coli
- gene expression
- cell proliferation
- type diabetes
- dna binding
- squamous cell carcinoma
- radiation therapy
- blood glucose
- high resolution
- locally advanced
- wastewater treatment
- metabolic syndrome
- skeletal muscle
- high fat diet induced
- weight loss
- anaerobic digestion
- genome wide analysis