The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells.
Claudia SolariMaría Victoria PetroneAyelén ToroCamila Vazquez EchegarayMaría Soledad CosentinoAriel WaismanMarcos FranciaLino BarañaoSantiago MiriukaAlejandra S GubermanPublished in: BMC research notes (2019)
We found that Gsr gene is expressed in ESCs during the pluripotent state and it was upregulated when these cells were induced to differentiate, concomitantly with Nanog decreased expression. Moreover, we found an increase in Gsr mRNA levels when Nanog was downregulated by a specific shRNA targeting this transcription factor in ESCs. Our results suggest that Nanog represses Gsr gene expression in ESCs, evidencing a role of this crucial pluripotency transcription factor in preservation of redox homeostasis in stem cells.
Keyphrases
- embryonic stem cells
- transcription factor
- gene expression
- stem cells
- genome wide identification
- dna methylation
- dna binding
- induced apoptosis
- poor prognosis
- genome wide
- cell cycle arrest
- diabetic rats
- long non coding rna
- cell therapy
- cell death
- signaling pathway
- cell proliferation
- endoplasmic reticulum stress
- pi k akt
- stress induced