Quantifying single-cell secretion in real time using resonant hyperspectral imaging.
Jose Juan-ColasIan S HitchcockMark ColesSteven JohnsonThomas F KraussPublished in: Proceedings of the National Academy of Sciences of the United States of America (2018)
Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.
Keyphrases
- single cell
- high throughput
- rna seq
- induced apoptosis
- cell cycle arrest
- label free
- binding protein
- high resolution
- protein protein
- immune response
- poor prognosis
- amino acid
- signaling pathway
- oxidative stress
- endoplasmic reticulum stress
- stem cells
- small molecule
- cell death
- mass spectrometry
- long non coding rna
- photodynamic therapy
- cell therapy
- energy transfer
- cell proliferation
- bone marrow
- high speed