Mn-Containing Bioactive Glass-Ceramics: BMP-2-Mimetic Peptide Covalent Grafting Boosts Human-Osteoblast Proliferation and Mineral Deposition.
Leonardo CassariPaola BrunMichele Di FoggiaPaola TaddeiAnnj ZamunerAntonella PasquatoAdriana De StefanisVeronica ValentiniMircea Vicentiu SaceleanuJulietta V RauMonica DettinPublished in: Materials (Basel, Switzerland) (2022)
The addition of Mn in bioceramic formulation is gaining interest in the field of bone implants. Mn activates human osteoblast (h-osteoblast) integrins, enhancing cell proliferation with a dose-dependent effect, whereas Mn-enriched glasses induce inhibition of Gram-negative or Gram-positive bacteria and fungi. In an effort to further optimize Mn-containing scaffolds' beneficial interaction with h-osteoblasts, a selective and specific covalent functionalization with a bioactive peptide was carried out. The anchoring of a peptide, mapped on the BMP-2 wrist epitope, to the scaffold was performed by a reaction between an aldehyde group of the peptide and the aminic groups of silanized Mn-containing bioceramic. SEM-EDX, FT-IR, and Raman studies confirmed the presence of the peptide grafted onto the scaffold. In in vitro assays, a significant improvement in h-osteoblast proliferation, gene expression, and calcium salt deposition after 7 days was detected in the functionalized Mn-containing bioceramic compared to the controls.
Keyphrases
- gram negative
- bone regeneration
- room temperature
- transition metal
- gene expression
- endothelial cells
- metal organic framework
- cell proliferation
- multidrug resistant
- tissue engineering
- mesenchymal stem cells
- drug delivery
- cell cycle
- quantum dots
- pluripotent stem cells
- soft tissue
- body composition
- high resolution
- pi k akt
- single cell
- breast reconstruction