In Silico Identification of lncRNAs Regulating Sperm Motility in the Turkey ( Meleagris gallopavo L.).
Jan Paweł JastrzębskiAleksandra LipkaMarta MajewskaKarol Gustaw MakowczenkoŁukasz PauksztoJoanna WisniewskaSławomir DorockiKrzysztof KozlowskiMariola SłowińskaPublished in: International journal of molecular sciences (2022)
Long non-coding RNAs (lncRNAs) are transcripts not translated into proteins with a length of more than 200 bp. LncRNAs are considered an important factor in the regulation of countless biological processes, mainly through the regulation of gene expression and interactions with proteins. However, the detailed mechanism of interaction as well as functions of lncRNAs are still unclear and therefore constitute a serious research challenge. In this study, for the first time, potential mechanisms of lncRNA regulation of processes related to sperm motility in turkey were investigated and described. Customized bioinformatics analysis was used to detect and identify lncRNAs, and their correlations with differentially expressed genes and proteins were also investigated. Results revealed the expression of 863 new/unknown lncRNAs in ductus deferens, testes and epididymis of turkeys. Moreover, potential relationships of the lncRNAs with the coding mRNAs and their products were identified in turkey reproductive tissues. The results obtained from the OMICS study may be useful in describing and characterizing the way that lncRNAs regulate genes and proteins as well as signaling pathways related to sperm motility.
Keyphrases
- genome wide analysis
- genome wide identification
- bioinformatics analysis
- network analysis
- gene expression
- long non coding rna
- poor prognosis
- transcription factor
- signaling pathway
- biofilm formation
- dna methylation
- genome wide
- escherichia coli
- cystic fibrosis
- mass spectrometry
- atomic force microscopy
- human health
- molecular dynamics simulations
- induced apoptosis
- endoplasmic reticulum stress