Purified exosome product enhances chondrocyte survival and regeneration by modulating inflammation and promoting chondrogenesis.
Guidong ShiZeling LongRodolfo E De la VegaAtta BehfarSteven L MoranChristopher EvansChunfeng ZhaoPublished in: Regenerative medicine (2022)
Aim: This study was to detect the effects of purified exosome product (PEP) on C28/I2 cells and chondrocytes derived from osteoarthritis patients. Materials & methods: Cell viability and apoptosis assays were used to detect the effect of PEP on cells. qRT-PCR and cell fluorescence assays were used to investigate the potential mechanism of PEP on cell chondrogenesis. Results: PEP was internalized by cells at a fast rate and enhanced cellular proliferation and migration while attenuating apoptosis. These findings reflect the fact that PEP can increase the expression of PCNA and reduce the expression of CASP3 / 7 / 9 and BAX . Conclusion: This study suggests an innovative strategy for chondrogenesis that could be applied to osteoarthritis repair in the future.
Keyphrases
- cell cycle arrest
- induced apoptosis
- endoplasmic reticulum stress
- oxidative stress
- cell death
- poor prognosis
- pi k akt
- stem cells
- signaling pathway
- single cell
- rheumatoid arthritis
- end stage renal disease
- ejection fraction
- cell therapy
- knee osteoarthritis
- cell proliferation
- peritoneal dialysis
- binding protein
- bone marrow
- risk assessment
- climate change
- prognostic factors
- human health
- quantum dots