Anticancer property of Hemp Bioactive Peptides in Hep3B liver cancer cells through Akt/GSK3β/β-catenin signaling pathway.
Lian-Hui WeiYan DongYu-Feng SunXue-Song MeiXue-Song MaJie ShiQing-Li YangYan-Ru JiZheng-Hai ZhangHu-Nan SunXing-Rong SunShu-Min SongPublished in: Food science & nutrition (2021)
Foodborne protein hydrolysates exhibit biological activity that may be therapeutic in a number of human disease settings. Hemp peptides (HP) generated by controlled hydrolysis of hemp proteins have a number of health benefits and are of pharmaceutical value. In the present study, we produce small molecular weight HP from hemp seed and investigate its anticancer properties in Hep3B human liver cancer cells. We demonstrate that HP treatment increased apoptosis, reduced cell viability, and reduced cell migration in Hep3B human liver cancer cells without affecting the normal liver cell line L02. We correlate these phenotypes with increased cellular ROS levels, upregulation of cleaved caspase 3 and Bad, and downregulation of antiapoptotic Bcl-2. HP treatment led to increased Akt and GSK-3β phosphorylation, with subsequent downregulation of β-catenin, suggesting β-catenin signaling modulation as a critical mechanism by which HP exhibits anticancer properties. Our findings suggest HP are of potential therapeutic interest for liver cancer treatment.
Keyphrases
- signaling pathway
- cell proliferation
- cell migration
- cell death
- pi k akt
- epithelial mesenchymal transition
- healthcare
- public health
- induced apoptosis
- endothelial cells
- oxidative stress
- cell cycle arrest
- endoplasmic reticulum stress
- climate change
- small molecule
- risk assessment
- poor prognosis
- combination therapy
- health information
- replacement therapy
- human health
- smoking cessation