Rapid Screening and Synthesis of Abiotic Synthetic Receptors for Selective Bacterial Recognition.
Shengnan ShaoShuang GaoYuan LiYongqin LvPublished in: ACS applied materials & interfaces (2023)
The major challenges that impede the preparation of abiotic synthetic receptors designed to feature selective bacterial recognition properties are the complexity, nonrobustness, and environmental adaptability of live microbes. Here, we describe a new rapid screening strategy to determine the optimal polymer formulation on 96-well plates and then produce abiotic synthetic receptors by imprinting the surface marker lipopolysaccharide (LPS) of Gram-negative bacteria. The resulting LPS-imprinted nanoparticles reveal remarkable affinity toward LPS with an equilibrium dissociation constant ( K D ) value of 10 -12 M and can distinguish and selectively recognize specific bacteria in whole blood at concentrations down to 10 cells/mL. The incorporation of gold nanorods into imprinted nanoparticles allows selective microbial inactivation based on photothermal treatment. We have also demonstrated that the imprinted nanoparticles with high affinity for bacteria could induce bacteria clustering, drive the expression of quorum-sensing-controlled signal molecules, and eventually enhance the productivity of the cell factory.
Keyphrases
- inflammatory response
- single cell
- anti inflammatory
- induced apoptosis
- poor prognosis
- lps induced
- genome wide identification
- toll like receptor
- arabidopsis thaliana
- climate change
- cell cycle arrest
- machine learning
- solid phase extraction
- rna seq
- photodynamic therapy
- walled carbon nanotubes
- stem cells
- molecular dynamics
- genome wide
- deep learning
- oxidative stress
- cell therapy
- binding protein
- cell death
- gold nanoparticles
- combination therapy
- long non coding rna
- dna methylation