NMNAT2-mediated NAD+ generation is essential for quality control of aged oocytes.
Xinghan WuFeifei HuJuan ZengLongsen HanDanhong QiuHaichao WangJuan GeXiaoyan YingQiang WangPublished in: Aging cell (2019)
Advanced maternal age has been reported to impair oocyte quality; however, the underlying mechanisms remain to be explored. In the present study, we identified the lowered NAD+ content and decreased expression of NMNAT2 protein in oocytes from old mice. Specific depletion of NMNAT2 in mouse oocytes disturbs the meiotic apparatus assembly and metabolic activity. Of note, nicotinic acid supplementation during in vitro culture or forced expression of NMNAT2 in aged oocytes was capable of reducing the reactive oxygen species (ROS) production and incidence of spindle/chromosome defects. Moreover, we revealed that activation or overexpression of SIRT1 not only partly prevents the deficient phenotypes of aged oocytes but also ameliorates the meiotic anomalies and oxidative stress in NMNAT2-depleted oocytes. To sum up, our data indicate a role for NMNAT2 in controlling redox homeostasis during oocyte maturation and uncover that NMNAT2- NAD+ -SIRT1 is an important pathway mediating the effects of maternal age on oocyte developmental competence.
Keyphrases
- oxidative stress
- reactive oxygen species
- quality control
- poor prognosis
- dna damage
- ischemia reperfusion injury
- binding protein
- cell proliferation
- risk factors
- mouse model
- transcription factor
- pregnant women
- big data
- body mass index
- electronic health record
- metabolic syndrome
- physical activity
- insulin resistance
- protein protein
- endoplasmic reticulum stress