Laser cleavable probes for in situ multiplexed glycan detection by single cell mass spectrometry.
Jing HanXi HuangHuihui LiuJiyun WangCaiqiao XiongZongxiu NiePublished in: Chemical science (2019)
Glycans binding on the cell surface through glycosylation play a key role in controlling various cellular processes, and glycan analysis at a single-cell level is necessary to study cellular heterogeneity and diagnose diseases in the early stage. Herein, we synthesized a series of laser cleavable probes, which could sensitively detect glycans on single cells and tissues by laser desorption ionization mass spectrometry (LDI-MS). This multiplexed and quantitative glycan detection was applied to evaluate the alterations of four types of glycans on breast cancer cells and drug-resistant cancer cells at a single-cell level, indicating that drug resistance may be related to the upregulation of glycan with a β-d-galactoside (Galβ) group and Neu5Aca2-6Gal(NAc)-R. Moreover, the glycan spatial distribution in cancerous and paracancerous human tissues was also demonstrated by MS imaging, showing that glycans are overexpressed in cancerous tissues. Therefore, this single-cell MS approach exhibits promise for application in studying glycan functions which are essential for clinical biomarker discovery and diagnosis of related diseases.
Keyphrases
- cell surface
- single cell
- mass spectrometry
- rna seq
- drug resistant
- high throughput
- high resolution
- liquid chromatography
- small molecule
- early stage
- gene expression
- multiple sclerosis
- ms ms
- high performance liquid chromatography
- multidrug resistant
- capillary electrophoresis
- breast cancer cells
- endothelial cells
- gas chromatography
- acinetobacter baumannii
- induced apoptosis
- living cells
- radiation therapy
- poor prognosis
- squamous cell carcinoma
- oxidative stress
- single molecule
- tandem mass spectrometry
- pseudomonas aeruginosa
- big data
- photodynamic therapy
- cystic fibrosis
- lymph node
- binding protein