Secondary Metabolites from Dendrobium nobile and Their Activities Induce Metabolites Apoptosis in OSC-19 Cells.
Yufan MengMaosheng ZhangYike FangJianwen YangMinjian DongCheng-Xin SunShiji XiaoPublished in: Molecules (Basel, Switzerland) (2023)
To identify potential drug candidates, secondary metabolites of Dendrobium nobile were performed. As a result, two previously undescribed phenanthrene derivatives with a spirolactone ring ( 1 and 2 ), along with four known compounds, N - trans -cinnamoyltyramine ( 3 ), N - trans - p -coumaroyltyramine ( 4 ), N - trans -feruloyltyramine ( 5 ), and moscatilin ( 6 ), were isolated from Dendrobium nobile . The structures of the undescribed compounds were elucidated using NMR spectroscopy, electronic circular dichroism (ECD) calculations, and extensive spectroscopic data analysis. The cytotoxic effects of compounds on human tongue squamous cells OSC-19 were determined using MTT at concentrations of 2.5 μM, 5 μM, 10 μM, and 20 μM. Compound 6 exhibited potent inhibitory activity against OSC-19 cells with an IC 50 of 1.32 μM. Migration assays and western blot assays demonstrated that compound 6 effectively inhibited migration by down-regulating MMP2 and MMP9 at concentrations of 0.5 μM and 1 μM. To investigate its effect on apoptosis, we performed AO/PI staining, flow cytometry, and WB experiments. The results showed that increasing concentrations led to increased red fluorescence, decreased green fluorescence, increased apoptosis rate, decreased expression of bcl-2, caspase 3, caspase 9, and parp proteins, and increased bax expression. Furthermore, the phosphorylation of JNK and P38 was activated, suggesting that compound 6 may induce apoptosis via the MAPK pathway.
Keyphrases
- induced apoptosis
- cell cycle arrest
- endoplasmic reticulum stress
- cell death
- oxidative stress
- pi k akt
- signaling pathway
- data analysis
- flow cytometry
- ms ms
- poor prognosis
- dna damage
- single molecule
- endothelial cells
- mass spectrometry
- density functional theory
- high resolution
- high grade
- induced pluripotent stem cells
- molecular docking
- molecular dynamics simulations
- climate change