Intratracheal Administration of Stem Cell Membrane-Cloaked Naringin-Loaded Biomimetic Nanoparticles Promotes Resolution of Acute Lung Injury.
Hua JinYue ZhaoYinlian YaoShilong FanRenxing LuoXin ShenYanyan WangJiang PiGonghua HuangPublished in: Antioxidants (Basel, Switzerland) (2024)
Cytokine storm and ROS overproduction in the lung always lead to acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) in a very short time. Effectively controlling cytokine storm release syndrome (CRS) and scavenging ROS are key to the prevention and treatment of ALI/ARDS. In this work, the naringin nanoparticles (Nar-NPs) were prepared by the emulsification and evaporation method; then, the mesenchymal stem cell membranes (CMs) were extracted and coated onto the surface of the Nar-NPs through the hand extrusion method to obtain the biomimetic CM@Nar-NPs. In vitro, the CM@Nar-NPs showed good dispersity, excellent biocompatibility, and biosafety. At the cellular level, the CM@Nar-NPs had excellent abilities to target inflamed macrophages and the capacity to scavenge ROS. In vivo imaging demonstrated that the CM@Nar-NPs could target and accumulate in the inflammatory lungs. In an ALI mouse model, intratracheal ( i.t. ) instillation of the CM@Nar-NPs significantly decreased the ROS level, inhibited the proinflammatory cytokines, and remarkably promoted the survival rate. Additionally, the CM@Nar-NPs increased the expression of M2 marker (CD206), and decreased the expression of M1 marker (F4/80) in septic mice, suggesting that the Nar-modulated macrophages polarized towards the M2 subtype. Collectively, this work proves that a mesenchymal stem cell membrane-based biomimetic nanoparticle delivery system could efficiently target lung inflammation via i.t. administration; the released payload inhibited the production of inflammatory cytokines and ROS, and the Nar-modulated macrophages polarized towards the M2 phenotype which might contribute to their anti-inflammation effects. This nano-system provides an excellent pneumonia-treated platform with satisfactory biosafety and has great potential to effectively deliver herbal medicine.
Keyphrases
- acute respiratory distress syndrome
- oxide nanoparticles
- cell death
- dna damage
- extracorporeal membrane oxygenation
- reactive oxygen species
- oxidative stress
- mouse model
- poor prognosis
- bone marrow
- lipopolysaccharide induced
- stem cells
- mesenchymal stem cells
- lps induced
- type diabetes
- high resolution
- drug delivery
- acute kidney injury
- intensive care unit
- photodynamic therapy
- mass spectrometry
- adipose tissue
- skeletal muscle
- case report
- binding protein
- walled carbon nanotubes
- respiratory failure
- high fat diet induced