TGF-β regulates Sca-1 expression and plasticity of pre-neoplastic mammary epithelial stem cells.
Ján RemšíkMarkéta PíckováOndřej VacekRadek FedrLucia BinóAleš HamplKarel SoučekPublished in: Scientific reports (2020)
The epithelial-mesenchymal plasticity, in tight association with stemness, contributes to the mammary gland homeostasis, evolution of early neoplastic lesions and cancer dissemination. Focused on cell surfaceome, we used mouse models of pre-neoplastic mammary epithelial and cancer stem cells to reveal the connection between cell surface markers and distinct cell phenotypes. We mechanistically dissected the TGF-β family-driven regulation of Sca-1, one of the most commonly used adult stem cell markers. We further provided evidence that TGF-β disrupts the lineage commitment and promotes the accumulation of tumor-initiating cells in pre-neoplastic cells.
Keyphrases
- stem cells
- single cell
- cell therapy
- induced apoptosis
- cancer stem cells
- transforming growth factor
- cell cycle arrest
- cell surface
- poor prognosis
- bone marrow
- blood brain barrier
- squamous cell carcinoma
- signaling pathway
- gene expression
- oxidative stress
- papillary thyroid
- mesenchymal stem cells
- dna methylation
- long non coding rna
- lymph node metastasis
- squamous cell