Interaction between DMRT1 and PLZF protein regulates self-renewal and proliferation in male germline stem cells.
Yudong WeiDonghui YangXiaomin DuXiuwei YuMengfei ZhangFurong TangFanglin MaNa LiChunling BaiGuangpeng LiJinlian HuaPublished in: Molecular and cellular biochemistry (2020)
Double sex and mab-3 related transcription factor 1 (DMRT1) encodes a double sex/mab-3 (DM) domain, which is the most conserved structure that involved in sex determination both in vertebrates and invertebrates. This study revealed important roles of DMRT1 in maintaining self-renewal of male germline stem cells (mGSCs). Our results showed that insufficient expression of DMRT1 in mice testes resulted in decreased number of spermatogonial cells and collapse of testicular niche in vivo. Self-renewal and proliferation of mGSCs were inhibited. Based on the bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (co-IP) assay, it was finally revealed that the interaction between DMRT1 and promyelocytic leukemia zinc finger (PLZF) protein was essential for maintaining self-renewal of mGSCs. Moreover, BTB domain of PLZF, DM and DMRT1 domain of DMRT1 were indispensable in mGSC, which were responsible for preserving the quantity of germ cells. Our research provided a new scientific basis for studying the mechanism of self-renewal and spermatogenesis in goat mGSCs.
Keyphrases
- stem cells
- induced apoptosis
- transcription factor
- signaling pathway
- cell cycle arrest
- poor prognosis
- binding protein
- single cell
- type diabetes
- endoplasmic reticulum stress
- cell therapy
- bone marrow
- oxidative stress
- cell death
- small molecule
- metabolic syndrome
- adipose tissue
- amino acid
- monoclonal antibody
- long non coding rna
- mesenchymal stem cells
- mass spectrometry
- pi k akt
- high resolution
- glycemic control