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Detection of antibodies neutralizing historical and emerging SARS-CoV-2 strains using a thermodynamically coupled de novo biosensor system.

Jason Z ZhangAndy Hsien-Wei YehAlexandra C WallsBasile I M WickyKaiti SprouseLaura A VanBlarganRebecca TregerAlfredo Quijano-RubioMinh N PhamJohn C KraftIan C HaydonWei YangMichelle DeWittCameron ChowLauren CarterMark H WenerLance StewartDavid VeeslerMichael S DiamondDavid Baker
Published in: bioRxiv : the preprint server for biology (2021)
With global vaccination efforts against SARS-CoV-2 underway, there is a need for rapid quantification methods for neutralizing antibodies elicited by vaccination and characterization of their strain dependence. Here, we describe a designed protein biosensor that enables sensitive and rapid detection of neutralizing antibodies against wild type and variant SARS-CoV-2 in serum samples. More generally, our thermodynamic coupling approach can better distinguish sample to sample differences in analyte binding affinity and abundance than traditional competition based assays.
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