SLC38A3 Promotes the Proliferation and Migration of Tumor Cells and Predicts Poor Prognosis in Colorectal Cancer.
Siyi ZhangLingli HuangYoujie ZengGe GaoHui WuDai LiRen GuoPublished in: ACS omega (2024)
Previous studies have revealed that abnormal expressions of membrane transporters were associated with colorectal cancer (CRC). We herein performed a comprehensive bioinformatics analysis to identify the key transporter protein-related genes involved in CRC and potential mechanisms. Differentially expressed transporter protein-related genes (DE-TPRGs) were identified from CRC and normal samples using The Cancer Genome Atlas database. SLC38A3 expression was validated by immunohistochemistry and RT-qPCR, and the potential mechanism was explored. A total of 63 DE-TPRGs (29 up-regulated and 34 down-regulated) were screened. Inside, ABCC2 , ABCG2 , SLC4A4 , SLC9A3 , SLC15A1 , and SLC38A3 were identified as hub genes. SLC38A3 is indeed upregulated in colorectal cancer patients. Furthermore, we found that knockdown of SLC38A3 inhibited the proliferation and migration of HCT116 cells, and Hsp70 ATPase activator could rescue it. Overall, SLC38A3 is a novel potential biomarker involved in CRC progression and promotes the proliferation and migration of tumor cells by positively regulating the function of Hsp70.
Keyphrases
- poor prognosis
- bioinformatics analysis
- long non coding rna
- heat shock protein
- cell cycle arrest
- heat shock
- single cell
- transcription factor
- heat stress
- protein protein
- binding protein
- genome wide
- amino acid
- human health
- gene expression
- nuclear factor
- squamous cell
- inflammatory response
- dna methylation
- endoplasmic reticulum
- genome wide identification
- lymph node metastasis
- genome wide analysis