Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome.

Jiawen CuiNan ShenZhaogeng LuGuolu XuYuyao WangBiao Jin

Published in: Plant methods (2020)

This paper made a comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome, the results indicate that ONT Pc is more cost-effective for generating extremely long reads and can characterise the transcriptome as well as quantify transcript expression. Therefore, ONT Pc is a new cost-effective and worthwhile method for full-length single-molecule transcriptome analysis in plants.

Keyphrases

single molecule
single cell
rna seq
transcription factor
atomic force microscopy
living cells
genome wide
gene expression
poor prognosis
cell wall
binding protein
dna methylation
long non coding rna
solid state
fluorescent probe