Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen.
Yezhen FanKui LiuRuoxi LuJieyu GaoWu SongHongyan ZhuXiaofeng TangYongsheng LiuMin MiaoPublished in: Journal of fungi (Basel, Switzerland) (2023)
Biological control of postharvest diseases has been proven to be an effective alternative to chemical control. As an environmentally friendly biocontrol agent, Bacillus subtilis has been widely applied. This study explores its application in kiwifruit soft rot and reveals the corresponding mechanisms. Treatment with cell-free supernatant (CFS) of Bacillus subtilis BS-1 significantly inhibits the mycelial growth of the pathogen Botryosphaeria dothidea and attenuates the pathogenicity on kiwifruit in a concentration-dependent manner. In particular, mycelial growth diameter was only 21% of the control after 3 days of treatment with 5% CFS. CFS caused swelling and breakage of the hyphae of B. dothidea observed by scanning electron microscopy, resulting in the leakage of nucleic acid and soluble protein and the loss of ergosterol content. Further analysis demonstrated that CFS significantly induces the expression of Nox genes associated with reactive oxygen species (ROS) production by 1.9-2.7-fold, leading to a considerable accumulation of ROS in cells and causing mycelial cell death. Our findings demonstrate that the biocontrol effect of B. subtilis BS-1 CFS on B. dothidea is realized by inducing oxidative damage to the mycelia cell.
Keyphrases
- bacillus subtilis
- cell free
- reactive oxygen species
- cell death
- electron microscopy
- oxidative stress
- nucleic acid
- dna damage
- cell cycle arrest
- circulating tumor
- induced apoptosis
- candida albicans
- single cell
- poor prognosis
- stem cells
- escherichia coli
- mesenchymal stem cells
- combination therapy
- high resolution
- amino acid
- small molecule
- binding protein
- mass spectrometry
- biofilm formation
- optic nerve
- heat stress
- replacement therapy