A new candidate oncogenic lncRNA derived from pseudogene WFDC21P promotes tumor progression in gastric cancer.
Huaiping CuiZhaoyu JiangShujie ZengHao WuZihao ZhangXiaobo GuoKangdi DongJinshen WangLiang ShangLeping LiPublished in: Cell death & disease (2021)
As oncogenes and tumor suppressor genes, long non-coding RNAs (lncRNAs) regulate the biological behavior of gastric cancer (GC) cells such as proliferation, invasion, and metastasis through various signal pathways. At present, although numerous lncRNAs that significantly influence the development and progression of GC have been identified, a considerable number of them have not been found and studied yet. In this study, we identified a new lncRNA derived from pseudogenes WFDC21P, which have not been reported in any previous GC study. LncRNA WFDC21P was significantly upregulated in GC cells and tissues, and clinically associated with the pathological stages of advanced GC. WFDC21P promoted proliferation and metastasis of GC cells both in vitro and in vivo. LncRNA WFDC21P was directly bound to GTPase Ran and it promoted the activity of the Akt/GSK3β/β-catenin pathway. Forkhead Box P3 (FOXP3), as a transcription factor of WFDC21P, was directly bound to the promoter region and it positively regulated the transcription of WFDC21P. This finding may provide a novel biomarker and therapeutic target for GC.
Keyphrases
- transcription factor
- long non coding rna
- induced apoptosis
- signaling pathway
- gas chromatography
- cell cycle arrest
- poor prognosis
- gene expression
- pi k akt
- long noncoding rna
- mass spectrometry
- endoplasmic reticulum stress
- oxidative stress
- cell death
- cell migration
- tandem mass spectrometry
- dendritic cells
- binding protein
- simultaneous determination